IFO50474 NOMO-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50474 Cell Name   NOMO-1
Profile   This cell line is useful for studying the differentiation and maturation of monocytes. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute monocytic leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   round Character   leukemia, acute monocytic, promonocytic leukemia cells
Classify   tumor Established by   Ogura, M. et al.
Registered by   Ogura,M. Regulation for Distribution   
Comment    Year   1998
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   Dilution, split ratio 1:4-1:6
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1609
Reference
Pubmed id:1845845FDP D-dimer induces the secretion of interleukin-1, urokinase-type plasminogen activator, and plasminogen activator inhibitor-2 in a human promonocytic leukemia cell line.
Hamaguchi M,Morishita Y,Takahashi I,Ogura M,Takamatsu J,Saito H
Blood. 1991 Jan 1;77(1):94-100
Pubmed id:1701357Up-regulation of transferrin receptor gene expression by granulocyte colony-stimulating factor in human myeloid leukemia cells.
Morishita Y,Kataoka T,Towatari M,Ito T,Inoue H,Ogura M,Morishima Y,Saito H
Cancer Res. 1990 Dec 15;50(24):7955-61
Pubmed id:1699657Enhanced expression of DNA topoisomerase II by recombinant human granulocyte colony-stimulating factor in human leukemia cells.
Towatari M,Ito Y,Morishita Y,Tanimoto M,Kawashima K,Morishima Y,Andoh T,Saito H
Cancer Res. 1990 Nov 15;50(22):7198-202

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50474 Cell Name   
LOT No.   01242007 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 484735) Temperature   37 C
Cell Density at Seeding   1.5 - 2.7 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   approx. 38 hrs Cell Number in Vial (cells/1ml)   8.1 x 10^6
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50474 Cell Name   NOMO-1
LOT No.   717 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50474 Cell Name   NOMO-1
LOT No.   11112014 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.4 - 1.6 x 106 Methods for Passages   Dilution, split ratio 1:4 - 1:6 (approx. 2 - 4 x 10^5 cells/mL)
Doubling Time    Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   93.4 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,13
D13S317:8,10
D7S820:8,10
D16S539:9,11
VWA:17,18
TH01:6,9
AM:X
TPOX:8,12
CSF1PO:11,12
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   93.4 Additional information   
Cell No.   IFO50474 Cell Name   NOMO-1
LOT No.   02012018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture), split ratio 1:4 - 1:6.
Doubling Time   approx. 37 hrs. Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   86 Additional information   
Cell No.   IFO50474 Cell Name   NOMO-1
LOT No.   04242020 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1 - 4 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 1.5 - 2 days Cell Number in Vial (cells/1ml)   6.6 x 10^6
Viability at cell freezing (%)   92.3 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   81.4 Additional information   
Cell No.   IFO50474 Cell Name   NOMO-1
LOT No.   08072023 Lot Specification   distribution
Medium   RPMI1640 medium (GIBCO,Cat No.11875) with 10% heat inactivated fetal bovine serum (SIGMA,　Cat#172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   2.0-2.49x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.07x10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:8,10
D7S820:8,10
D16S539:9,11
VWA:17,18
TH01:6,9
AM:X
TPOX:8,12
CSF1PO:11,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   CELLBANKER1((Nihon Zenyaku Industries) CO2 Conc.   5%
Viability immediately after thawing (%)   93 Additional information   
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