IFO50444 KNS-81-FD

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50444 Cell Name   KNS-81-FD
Profile   GFAP-positive human glioma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   65
Identity   available Tissue for Primary Cancer   neural
Case history   glioma Metastasis   
Tissue Metastasized    Genetics   serum-free adapted subline of KNS-81 (IFO 50359)
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   glioma, derivative of KNS-81 adapted to serum-free medium
Classify   variant Established by   Takeshita,I.
Registered by   Takeshita,I. Regulation for Distribution   
Comment   The DNA fingerprint profile confirmed that this cell line derives from KNS-81. Year   1994
Medium   1 : 1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium. Serum-free Methods for Passages   100 USP units/ml crystalline trypsin, 100 protease units/ml dispase and 0.02% trypsin in Ca, Mg-free PBS
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1336
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50444 Cell Name   
LOT No.   03092005 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium. Serum-free. Temperature   37 C
Cell Density at Seeding   5 - 10 x 10^4 cells/ml Methods for Passages   100 USP units/ml crystelline trypsin, 100 PU/ml dispase and 0.02% EDTA. Trypsin action was stopped with trypsin inhibitor. Split 1:1
Doubling Time   Very slow (more than 1 week). Cell Number in Vial (cells/1ml)   7.6 x 10^5
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   P27 after serum-free PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   0.1% methylcellulose, 10% DMSO - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50444 Cell Name   KNS-81-FD
LOT No.   948 Lot Specification   seed/distribution
Medium   Ham'sF12 : D.MEM = 1:1 mixture, serum-free Temperature   37 C
Cell Density at Seeding    Methods for Passages   100 USP units/ml crystalline trypsin, 100 protease units/ml dispase & 0.02% EDTA-PBS
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   P20 after serum-free PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,12
D13S317:9
D7S820:11
D16S539:10,12
VWA:17,18
TH01:7,9
AM:XY
TPOX:8,11
CSF1PO:9,12
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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