IFO50415 C3H/10T1/2-clone8

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50415 Cell Name   C3H/10T1/2-clone8
Profile   This cell line is useful for transformation studies. Other Name   
Animal   mouse Strain   C3H
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   fibroblast, contact-inhibited
Classify   normal Established by   Reznikoff, C. A. et al.
Registered by   Iwasa,S. Regulation for Distribution   
Comment    Year   1993
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0190
Reference
Pubmed id:1277196In vitro correlates of transformation in C3H/10T1/2 clone 8 mouse cells.
Jones PA,Laug WE,Gardner A,Nye CA,Fink LM,Benedict WF
Cancer Res. 1976 Aug;36(8):2863-7
Pubmed id:129280Oncogenic transformation of C3H/10T1/2 clone 8 mouse embryo cells by halogenated pyrimidine nucleosides.
Jones PA,Benedict WF,Baker MS,Mondal S,Rapp U,Heidelberger C
Cancer Res. 1976 Jan;36(1):101-7
Pubmed id:1167940Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation.
Terzaghi M,Little JB
Nature. 1975 Feb 13;253(5492):548-9
Pubmed id:4812259Cell cycle dependency of oncogenic transformation induced by N-methyl-N'-nitro-N-nitrosoquanidine in culture.
Bertram JS,Heidelberger C
Cancer Res. 1974 Mar;34(3):526-37
Pubmed id:4796800Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division.
Reznikoff CA,Bertram JS,Brankow DW,Heidelberger C
Cancer Res. 1973 Dec;33(12):3239-49




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50415 Cell Name   C3H/10T1/2-clone8
LOT No.   1157 Lot Specification   distribution
Medium   Eagle's basal medium, 90%; FBS, 10% Temperature   37 C
Cell Density at Seeding   1.5 x 10^4 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   5.7 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P18 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, PEP B, AST, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   Lot# is labeled as "R843F1157" on vials.
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