IFO50410 VERO 76

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50410 Cell Name   VERO 76
Profile   Subclone of the VERO cells. Other Name   
Animal   monkey, African green Strain   African green
Genus   Cercopithecus Species   aethiops
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epitherial like Character   subclone of Vero cells
Classify   variant Established by   Johnson, K. M.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1993
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0603
Reference
Pubmed id:2725683Mycoplasma infection of cultured cells.
Hay RJ,Macy ML,Chen TR
Nature. 1989 Jun 8;339(6224):487-8
Pubmed id:4290661SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters.
Girardi AJ,Weinstein D,Moorhead PS
Ann Med Exp Biol Fenn. 1966;44(2):242-54
Pubmed id:5833688Studies on measles virus. II. Propagation in two established simian renal cell lines and development of a plaque assay.
Sasaki K,Makino S,Kasahara S
Kitasato Arch Exp Med. 1964 Dec;37(1):27-42

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50410 Cell Name   
LOT No.   07252001 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   7.6 x 10^5
Viability at cell freezing (%)   86.8 Antibiotics Used   free
Passage Number   P2* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as African green monkey by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50410 Cell Name   VERO 76
LOT No.   879 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, MD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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