IFO50376 MSS62

Cell information

Important Notice(s)

RITC80-7 medium

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50376 Cell Name   MSS62
Profile   spleen stromal cell line, (C57BL/6 mouse) Other Name   
Animal   mouse Strain   C57BL/6
Genus   Mus Species   musculus
Sex    Age   newborn
Identity    Tissue for Primary Cancer   spleen
Case history   spleen stromal cell Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   spleen stromal cell line
Classify    Established by   Yanai, N. et al.
Registered by   Yanai,N. Regulation for Distribution   
Comment    Year   1992
Medium   RITC80-7 with 2% fetal calf serum, 10ng/ml EGF, 5ul/L monoethanolamine,10ug/ml Tf & 1ug/ml insulin Methods for Passages   25 ug/ml pronase-Hanks'BSS
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   spleen
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5563
Reference
Pubmed id:2032312Endothelial cells create a hematopoietic inductive microenvironment preferential to erythropoiesis in the mouse spleen.
Yanai N,Satoh T,Obinata M
Cell Struct Funct. 1991 Feb;16(1):87-93
Pubmed id:2804371Spleen stromal cell lines selectively support erythroid colony formation.
Yanai N,Matsuya Y,Obinata M
Blood. 1989 Nov 15;74(7):2391-7




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50376 Cell Name   MSS62
LOT No.   1022 Lot Specification   distribution
Medium   RITC80-7 with 2%FCS, 10ng/ml EGF, 5 ul/L monoethanolamine, 10 ug/ml Tf, 1 ug/ml insulin Temperature   37 C
Cell Density at Seeding    Methods for Passages   25 ug/ml pronase-Hanks'BSS
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^5
Viability at cell freezing (%)   80 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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