IFO50374 TKD2

Cell information

Important Notice(s)

RITC80-7 medium

Growth temperature of this cell line is 33 degree centigrade !!

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50374 Cell Name   TKD2
Profile   Acetylated low-densitiy lipoprotein is incorporated into the cells. Temperature sensitive cell line. Growth temperature is 33 C. Other Name   
Animal   mouse Strain   C57BL/6 transgenic
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   vascular system
Case history   endothelial cell Metastasis   
Tissue Metastasized    Genetics   TG mouse harboring tsSV40 large T antigen gene
Life Span   infinite Crisis PDL   
Morphology    Character   endothelial cell, tsSV40 large T-immortalized. Growth temperature is 33 C.
Classify    Established by   Yanai, N. et al.
Registered by   Yanai,N. Regulation for Distribution   
Comment    Year   1992
Medium   RITC80-7 with 2% fetal calf serum, 10ng/ml EGF, 5ul/L monoethanolamine,10ug/ml Tf & 1ug/ml insulin Methods for Passages   25 ug/ml pronase-Hanks'BSS
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5598
Reference
Pubmed id:1663919A tubule cell line established from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.
Yanai N,Satoh T,Kyo S,Abe K,Suzuki M,Obinata M
Jpn J Cancer Res. 1991 Dec;82(12):1344-8




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50374 Cell Name   TKD2
LOT No.   04152002 Lot Specification   distribution
Medium   RITC80-7 medium with 10 ng/ml EGF, 5 ul/L monoethanolamine, 10 ug/ml transferrin, 1 ug/ml insulin and 2% FCS Temperature   33 C!!
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   25 ug/ml pronase in Hanks' balanced salt solution
Doubling Time   D.T. = ca 33 hrs Cell Number in Vial (cells/1ml)   ca 2 x 10^6
Viability at cell freezing (%)   88.7 Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FCS - RITC80-7 CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50374 Cell Name   TKD2
LOT No.   857 Lot Specification   seed/distribution
Medium   RITC80-7 with 2%FCS, 10ng/ml EGF, 5 ul/L monoethanolamine, 10 ug/ml Tf, 1 ug/ml insulin Temperature   33 C
Cell Density at Seeding    Methods for Passages   25 ug/ml pronase-Hanks'BSS
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   tsSV40 large T antigen gene
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50374 Cell Name   TKD2
LOT No.   05212013 Lot Specification   distribution
Medium   RITC80-7 with 2% fetal calf serum, 10ng/ml EGF, 5ug/L monoethanolamine,10ug/ml Tf & 1ug/ml insulin Temperature   33 C
Cell Density at Seeding   2 - 3.2 x 10^4 cells / mL Methods for Passages   25 ug/ml pronase-Hanks'BSS
Doubling Time    Cell Number in Vial (cells/1ml)   8.6 x 10^5 cells / m
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   p16 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% FBS, RITC80-7 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50374 Cell Name   TKD2
LOT No.   02252021 Lot Specification   distribution
Medium   RITC80-7 medium with 2% fetal bovime serum (FBS; Sigma Cat. # 172012), 10 ng/ml EGF, 5 ug/L monoethanolamine, 10 ug/ml transferrin & 1 ug/ml insulin Temperature   33 C !!
Cell Density at Seeding   0.5 - 3.8 x 10^5 cells/mL Methods for Passages   25 ug/ml pronase in Hanks' balanced salt solution
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   8.6 x 10^5
Viability at cell freezing (%)   84.6 Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RITC80-7 medium CO2 Conc.   5%
Viability immediately after thawing (%)   88.4 Additional information   
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