IFO50373 TKC2

Cell information

Important Notice(s)

RITC80-7 medium

Growth temperature of this cell line is 33 degree centigrade !!

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50373 Cell Name   TKC2
Profile   cAMP synthesis is stimulated by arginine vasopressin in the cells. Growth temperature is 33 C. Other Name   
Animal   mouse Strain   C57BL/6 transgenic
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history   tuble cell Metastasis   
Tissue Metastasized    Genetics   TG mouse harboring tsSV40 large T antigen gene
Life Span   infinite Crisis PDL   
Morphology    Character   tubule cell, tsSV40 large T-immortalized (ts mutant)
Classify   mutant Established by   Yanai, N. et al.
Registered by   Yanai,N. Regulation for Distribution   
Comment    Year   1992
Medium   RITC80-7 with 2% fetal calf serum, 10ng/ml EGF, 5ul/L monoethanolamine,10ug/ml Tf & 1ug/ml insulin. Ts cell line (33 C). Methods for Passages   25 ug/ml pronase-Hanks'BSS
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5597
Reference
Pubmed id:1663919A tubule cell line established from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.
Yanai N,Satoh T,Kyo S,Abe K,Suzuki M,Obinata M
Jpn J Cancer Res. 1991 Dec;82(12):1344-8




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50373 Cell Name   TKC2
LOT No.   04192002 Lot Specification   distribution
Medium   RITC80-7 medium with 10 ng/ml EGF, 10 ug/ml transferrin, 1 ug/ml insulin, 5 ul/L monoethanolamine and 2% FBS Temperature   33 C !!
Cell Density at Seeding   1.0 x 10^5 cells/ml Methods for Passages   25 ug/ml pronase in Hanks' BSS
Doubling Time   D.T. = ca 27 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   82.9 Antibiotics Used   free
Passage Number   P15 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50373 Cell Name   TKC2
LOT No.   874 Lot Specification   seed/distribution
Medium   RITC80-7 with 2%FCS, 10ng/ml EGF, 5 lg/L monoethanolamine, 10 ug/ml Tf, 1 ug/ml insulin. Temperature   33 C
Cell Density at Seeding    Methods for Passages   25 ug/ml pronase-Hanks'BSS
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   free, pretreatment with MC210
Passage Number   >8 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, PepB, LD
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   tsSV40 large T antigen gene
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50373 Cell Name   TKC2
LOT No.   03172021 Lot Specification   distribution
Medium   RITC80-7 medium with 10 ng/ml EGF, 10 ug/ml transferrin, 1 ug/ml insulin, 5 ul/L monoethanolamine and 2% FBS (Sigma Cat. # 172012, Lot 12J396) Temperature   33 C !!
Cell Density at Seeding   4.8 - 7.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 25 ug/mL pronase in Hanks' BSS.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   9.3 x 10^5
Viability at cell freezing (%)   91.5 Antibiotics Used   free
Passage Number   P11 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RITC80-7 medium CO2 Conc.   5%
Viability immediately after thawing (%)   96.7 Additional information   
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