IFO50368 no.10

Cell information

Important Notice(s)

本細胞株の分譲に際しての寄託者の承諾について (On the distribution of this cell line. An approval of depositor is required.)

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50368 Cell Name   no.10
Profile   Human anaplastic glioma cell line derived from brain. Other Name   Onda 10
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   62
Identity   available Tissue for Primary Cancer   neural
Case history   anaplastic glioma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epitherial like Character   glioma, anaplastic, GFAP was first positive, but was lost at 100 passages. This cell line around 200 passage was deposited at cell bank.
Classify   tumor Established by   Onda, K. et al.
Registered by   Washiyama,K. Regulation for Distribution   Restricted: Cell's developer's approval is required
Comment    Year   1992
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3075
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 908452
Reference
Pubmed id:3188836Correlation of DNA ploidy and morphological features of human glioma cell cultures with the establishment of cell lines.
Onda K,Tanaka R,Washiyama K,Takeda N,Kumanishi T
Acta Neuropathol. 1988;76(5):433-40

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50368 Cell Name   
LOT No.   08082001 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   93.5 Antibiotics Used   free
Passage Number   P205 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:12
D7S820:8,11
D16S539:11
VWA:17
TH01:7,9
AM:X,Y
TPOX:9
CSF1PO:12,13
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50368 Cell Name   no.10
LOT No.   854 Lot Specification   seed/distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P202 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:12
D7S820:8,11
D16S539:11
VWA:17
TH01:7,9
AM:XY
TPOX:9
CSF1PO:12,13
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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Cell No.   IFO50368 Cell Name   no.10
LOT No.   07052024 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 52 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   P207 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:12
D7S820:8,11
D16S539:11
VWA:17
TH01:7,9
AM:X,Y
TPOX:9
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90.2 Additional information   
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