IFO50367 SHOK

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50367 Cell Name   SHOK
Profile   This cell line is useful for transformation assay. Other Name   
Animal   hamster, Syrian Strain   Syrian
Genus   Mesocricetus Species   auratus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   fibroblast, contact-inhibited
Classify   normal Established by   Higashi, T.
Registered by   Tatsuka,M. Regulation for Distribution   
Comment    Year   1992
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryo, whole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3164
Reference
Pubmed id:2181436Hamster cell line suitable for transfection assay of transforming genes.
Higashi T,Sasai H,Suzuki F,Miyoshi J,Ohuchi T,Takai S,Mori T,Kakunaga T
Proc Natl Acad Sci U S A. 1990 Apr;87(7):2409-13

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50367 Cell Name   SHOK
LOT No.   1162 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   2-5 x 10^5 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   6.2 x 10^6
Viability at cell freezing (%)   93.3 Antibiotics Used   free
Passage Number   Unknown (9 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, AST, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   Lot# is labelled as R822F1162.
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