IFO50364 L6
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50364 | Cell Name | L6 |
|---|---|---|---|
| Profile | skeletal muscle myoblast | Other Name | |
| Animal | rat | Strain | |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | muscular system | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | skeletal muscle myoblast |
| Classify | Established by | Yaffe, D. et al. | |
| Registered by | Sasada,R. | Regulation for Distribution | |
| Comment | Year | 1991 | |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | skeletal myoblast |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:2533272 | Spectinomycin operon of Micrococcus luteus: evolutionary implications of organization and novel codon usage. Ohama T,Muto A,Osawa S J Mol Evol. 1989 Nov;29(5):381-95 |
| Pubmed id:6336315 | A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. Yanagi Y,Yoshikai Y,Leggett K,Clark SP,Aleksander I,Mak TW Nature. 1984 Mar 8-14;308(5955):145-9 |
| Pubmed id:5481965 | The in vitro cultivation and differentiation capacities of myogenic cell lines. Richler C,Yaffe D Dev Biol. 1970 Sep;23(1):1-22 |
| Pubmed id:5245982 | Retention of differentiation potentialities during prolonged cultivation of myogenic cells. Yaffe D Proc Natl Acad Sci U S A. 1968 Oct;61(2):477-83 |
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | IFO50364 | Cell Name | |
|---|---|---|---|
| LOT No. | 01262010 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 5.4 - 7.3 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA. Passage twice a week. |
| Doubling Time | approx. 22 hrs | Cell Number in Vial (cells/1ml) | 3.1 x 10^6 |
| Viability at cell freezing (%) | 91.0 | Antibiotics Used | free |
| Passage Number | P28 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as rat by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50364 | Cell Name | L6 |
|---|---|---|---|
| LOT No. | 1128 | Lot Specification | distribution |
| Medium | D.MEM, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | 1-2 x 10^5 cells/ml | Methods for Passages | trypsin-EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 3.6 x 10^6 | |
| Viability at cell freezing (%) | 83 | Antibiotics Used | free |
| Passage Number | P27 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD, PepB |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.)-culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |