-Detailed Information [IFO50355]-

IFO50355 ONS-76

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	IFO50355	Cell Name	ONS-76
Profile	medulloblastoma	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age	2
Identity	available	Tissue for Primary Cancer	neural
Case history	medulloblastoma	Metastasis
Tissue Metastasized		Genetics
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	medulloblastoma
Classify	tumor	Established by	Shimizu, K.
Registered by	Shimizu,K.	Regulation for Distribution
Comment		Year	1991
Medium	RPMI1640 medium with 10% fetal bovine serum	Methods for Passages	trypsin-EDTA
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	brain
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1624

Reference
Pubmed id:2504489	Expression of major histocompatibility complex on human medulloblastoma cells with neuronal differentiation. Tamura K,Shimizu K,Yamada M,Okamoto Y,Matsui Y,Park KC,Mabuchi E,Moriuchi S,Mogami H Cancer Res. 1989 Oct 1;49(19):5380-4

Images

Movies

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

08192008
1000
12042014
10262020
08292024

Cell No.	IFO50355	Cell Name
LOT No.	08192008	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	5 - 7 x 10^4 cells/mL	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 24 hrs	Cell Number in Vial (cells/1ml)	3.7 x 10^6
Viability at cell freezing (%)	96.2	Antibiotics Used	free
Passage Number	P58	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50355	Cell Name	ONS-76
LOT No.	1000	Lot Specification	distribution
Medium	RPMI 1640, 90% ; FBS, 10%	Temperature	37 C
Cell Density at Seeding		Methods for Passages	trypsin-EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	3 x 10^6
Viability at cell freezing (%)	92	Antibiotics Used	free
Passage Number	P57	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NP, G6PD(type B), MD
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% serum (final conc.) - culture medium	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	IFO50355	Cell Name	ONS-76
LOT No.	12042014	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	4.0 - 5.3 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 32 hrs	Cell Number in Vial (cells/1ml)	8.2 x 10^5
Viability at cell freezing (%)	90	Antibiotics Used	free
Passage Number	P57	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	90	Additional information

Cell No.	IFO50355	Cell Name	ONS-76
LOT No.	10262020	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.8 - 1.8 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 1 day	Cell Number in Vial (cells/1ml)	2.4 x 10^6
Viability at cell freezing (%)	97.2	Antibiotics Used	free
Passage Number	P57	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	94.4	Additional information

Cell No.	IFO50355	Cell Name	ONS-76
LOT No.	08292024	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	0.9 - 3.8 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 1 day	Cell Number in Vial (cells/1ml)	1.8 x 10^6
Viability at cell freezing (%)	98.9	Antibiotics Used	free
Passage Number	P57	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:9,10 D13S317:8,13 D7S820:11,12 D16S539:9,10 VWA:14,16 TH01:9 AM:X TPOX:8 CSF1PO:10,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	98.3	Additional information