IFO50322 NJG

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50322 Cell Name   NJG
Profile   gestational uterine choriocarcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   uterus
Case history   gestational uterine choriocarcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   gestational uterine choriocarcinoma
Classify   tumor Established by   Nozawa, S.
Registered by   Nozawa,S. Regulation for Distribution   
Comment   This cell line does not contain a DNA sequence for E6 oncogene of HPV type 16, 18 and 33. (Tested at IFO (PCR)). Year   1991
Medium   Ham's F12 medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   uterus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3073
Reference
Pubmed id:9125195Presence of mRNAs encoding the soluble D-factor/LIF receptor in human choriocarcinoma cells and production of the soluble receptor.
Tomida M
Biochem Biophys Res Commun. 1997 Mar 17;232(2):427-31
Pubmed id:3611867[Establishment and characteristics of a new human choriocarcinoma cell line and its production of tumor markers].
Nozawa S,Udagawa Y,Sakayori M,Tsukazaki K,Takayama Y,Aoki D,Kubushiro K,Iizuka R,Inaba N
Nihon Sanka Fujinka Gakkai Zasshi. 1987 Jun;39(6):889-96

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50322 Cell Name   
LOT No.   06132007 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   2-5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   34 hrs Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   Unknown (11 at cellbank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:11
D7S820:10
D16S539:12
VWA:16
TH01:9
AM:X
TPOX:8
CSF1PO:13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50322 Cell Name   NJG
LOT No.   1015 Lot Specification   distribution
Medium   Ham's F12, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   8 x 10^5
Viability at cell freezing (%)   80 Antibiotics Used   free
Passage Number   Unknown (>7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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