IFO50321 GAK
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50321 | Cell Name | GAK |
|---|---|---|---|
| Profile | Human malignant melanoma cell line with the production of 5-S-cysteinyldopa. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 86 |
| Identity | available | Tissue for Primary Cancer | melanoma |
| Case history | malignant melanoma | Metastasis | Yes |
| Tissue Metastasized | inguinal lymph node | Genetics | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epitherial like | Character | malignant melanoma, metastasis to lymph node. 5-S-cysteinyldopa production. |
| Classify | tumor | Established by | Nozawa, S. |
| Registered by | Nozawa,S. | Regulation for Distribution | |
| Comment | Year | 1991 | |
| Medium | Ham's F12 medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5 % | Tissue Sampling | inguinal lymph node |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:6431036 | [A newly established melanoma cell line (GAK) with 5-S-cysteinyldopa phenotype]. Nozawa S,Ohta H,Tsukazaki K,Udagawa Y,Kurihara S,Kano S Nihon Sanka Fujinka Gakkai Zasshi. 1984 Jun;36(6):883-92 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | IFO50321 | Cell Name | |
|---|---|---|---|
| LOT No. | 07202012 | Lot Specification | distribution |
| Medium | Ham's F12 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 3.6 - 7.3 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 55 hrs | Cell Number in Vial (cells/1ml) | 1.5 x 10^6 |
| Viability at cell freezing (%) | 80.2 | Antibiotics Used | free |
| Passage Number | Unknown (15 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - Ham's F12 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50321 | Cell Name | GAK |
|---|---|---|---|
| LOT No. | 1103 | Lot Specification | distribution |
| Medium | Ham's F12, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 3 x 10^5 | |
| Viability at cell freezing (%) | 72 | Antibiotics Used | free |
| Passage Number | Unknown (14 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD (type B), MD, AST |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:10 D13S317:9,13 D7S820:11 D16S539:12 VWA:15,18 TH01:7 AM:X TPOX:8,9 CSF1PO:10 |
|
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information | Lot# is labeled as R708F1103. |