IFO50314 SKN

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50314 Cell Name   SKN
Profile   leiomyosarcoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   uterus
Case history   uterine leiomyosarcoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epitherial like Character   leiomyosarcoma
Classify   tumor Established by   Nozawa, S.
Registered by   Nozawa,S. Regulation for Distribution   
Comment   This cell line does not contain a DNA sequence for E6 oncogene of HPV type 16, 18 and 33. (Tested at IFO (PCR)). Year   1991
Medium   Ham's F12 medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   uterus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3167
Reference
Pubmed id:556976Establishment of a human leiomyosarcoma cell line.
Ishiwata I,Nozawa S,Nagai S,Kurihara S,Mikata A
Cancer Res. 1977 Mar;37(3):658-64
Pubmed id:noneEstablishment and characterization of a human leiomyosarcoma cell line
Ishiwata,I., Nozawa,S., Kiguchi,K., Inoue,T., and Kurihara,S.
Acta Obst. Gynaec. Jpn., 30: 373-380, 1978.

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50314 Cell Name   SKN
LOT No.   1116 Lot Specification   distribution
Medium   Ham's F12, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (15 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,G6PD (type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:11
D7S820:8,12
D16S539:9
VWA:19
TH01:8
AM:X
TPOX:8
CSF1PO:10,11
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   Lot number is labeled as R701F1116 on vial.
Cell No.   IFO50314 Cell Name   SKN
LOT No.   07202018 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   2 - 8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 35 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   83 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)   83 Additional information   
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