IFO50310 SKG-IIIa
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50310 | Cell Name | SKG-IIIa |
|---|---|---|---|
| Profile | cervical epidermoid carcinoma, clear cell type | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | |
| Identity | available | Tissue for Primary Cancer | uterine cervix |
| Case history | uterine cervical epidermoid cancer | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | Character | cervical epidermoid cancer | |
| Classify | tumor | Established by | Nozawa, S. |
| Registered by | Nozawa,S. | Regulation for Distribution | |
| Comment | This cell line and SKG-IIIb (IFO 50311) originated from the same patient. This cell line contains a DNA sequence for E6 oncogene of HPV type 16, but not type 18 and 33. (Tested at IFO (PCR)). | Year | 1991 |
| Medium | Ham's F12 medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | uterine cervix |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | Integration of HPV16 to host genome (~one copy/cell) was reported (Int J Cancer 1986;37:499) | ||||||||
| Reference | |
|---|---|
| Pubmed id:6699456 | [Biological properties of two newly established cell lines (SKG-3a,3b) from a human uterine cervical epidermoid carcinoma]. Udagawa Y,Nozawa S,Tsukazaki K,Ohta H,Akiba T,Kurihara S Nihon Sanka Fujinka Gakkai Zasshi. 1984 Feb;36(2):237-46 |
| Pubmed id:6604031 | Sodium butyrate produces concordant expression of "early placental" alkaline phosphatase, pregnancy-specific beta 1-glycoprotein and human chronic gonadotropin beta-subunit in a newly established uterine cervical cancer cell line (SKG-IIIa). Nozawa S,Engvall E,Kano S,Kurihara S,Fishman WH Int J Cancer. 1983 Sep 15;32(3):267-72 |
| Pubmed id:6600963 | Newly established uterine cervical cancer cell line (SKG-III) with Regan isoenzyme, human chorionic gonadotropin beta-subunit, and pregnancy-specific beta 1-glycoprotein phenotypes. Nozawa S,Udagawa Y,Ohta H,Kurihara S,Fishman WH Cancer Res. 1983 Apr;43(4):1748-60 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | IFO50310 | Cell Name | SKG-IIIa |
|---|---|---|---|
| LOT No. | 1004 | Lot Specification | distribution |
| Medium | Ham's F12, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 5 x 10^6 | |
| Viability at cell freezing (%) | 89 | Antibiotics Used | free |
| Passage Number | Unknown (13 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD(type B), MD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information | Lot# is labeled as R697F1004 on vials |