IFO50285 U-251 MG (KO)
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50285 | Cell Name | U-251 MG (KO) |
|---|---|---|---|
| Profile | Subline of U-251 MG (IFO 50288) with epithelial morphology. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 75 |
| Identity | available | Tissue for Primary Cancer | neural |
| Case history | astrocytoma | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | astrocytoma, GFAP-positive |
| Classify | tumor | Established by | Bigner, D. D. |
| Registered by | Kaji,K. | Regulation for Distribution | |
| Comment | The DNA fingerprint profile confirmed that this cell line derives from the same origin as U-251 MG. | Year | 1990 |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | brain |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:6260907 | Heterogeneity of Genotypic and phenotypic characteristics of fifteen permanent cell lines derived from human gliomas. Bigner DD,Bigner SH,Pontén J,Westermark B,Mahaley MS,Ruoslahti E,Herschman H,Eng LF,Wikstrand CJ J Neuropathol Exp Neurol. 1981 May;40(3):201-29 |
| Pubmed id:283420 | Serological analysis of cell surface antigens of malignant human brain tumors. Pfreundschuh M,Shiku H,Takahashi T,Ueda R,Ransohoff J,Oettgen HF,Old LJ Proc Natl Acad Sci U S A. 1978 Oct;75(10):5122-6 |
| Pubmed id:359950 | Properties of human malignant glioma cells in vitro. Pontén J,Westermark B Med Biol. 1978 Aug;56(4):184-93 |
| Pubmed id:4359449 | Determinants for the establishment of permanent tissue culture lines from human gliomas. Westermark B,Pontén J,Hugosson R Acta Pathol Microbiol Scand A. 1973 Nov;81(6):791-805 |
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| PDF File1 |
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | IFO50285 | Cell Name | U-251 MG (KO) |
|---|---|---|---|
| LOT No. | 1003 | Lot Specification | distribution |
| Medium | E.MEM, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 4 x 10^5 | |
| Viability at cell freezing (%) | 89 | Antibiotics Used | free |
| Passage Number | Unknown (8 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD(type B), MD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:11 D13S317:10,11 D7S820:10,12 D16S539:12 VWA:16,18 TH01:10 AM:X TPOX:8 CSF1PO:11,12 |
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| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | IFO50285 | Cell Name | U-251 MG (KO) |
|---|---|---|---|
| LOT No. | 07302015 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | 1.7 - 4.8 x 10^4 cellls/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 23 hrs | Cell Number in Vial (cells/1ml) | 1.2 x 10^6 |
| Viability at cell freezing (%) | 95.1 | Antibiotics Used | free |
| Passage Number | Unknown (9 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:11,12 D13S317:10,11 D7S820:10,12 D16S539:12 VWA:16,18 TH01:9.3 AM:X,Y TPOX:8 CSF1PO:11,12 |
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| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 95.1 | Additional information |