IFO50284 A2B5 clone 105

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50284 Cell Name   A2B5 clone 105
Profile   Produces anti-chick retinal neuron glycolipid monoclonal antibody (IgM class). Other Name   
Animal   mouse Strain   
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   antibody-producing hybridoma
Case history   hybridoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   round Character   A2B5 antibody, recognizing a glycolipid expressed in neural tissue
Classify   hybridoma Established by   Eisenbarth, G. S.
Registered by   Igarashi,K. Regulation for Distribution   
Comment    Year   1990
Medium   Dulbecco's modified Eagle's medium (high glucose) with 5% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   P3x63 Ag8 x spleen
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_7946
Reference
Pubmed id:388422Monoclonal antibody to a plasma membrane antigen of neurons.
Eisenbarth GS,Walsh FS,Nirenberg M
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4913-7

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50284 Cell Name   A2B5 clone 105
LOT No.   973 Lot Specification   distribution
Medium   D.MEM (high glucose), 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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