IFO50282 RAT-2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50282 Cell Name   RAT-2
Profile   thymidine kinase deficient RAT-1 (F2408) cell line, (Fischer rat) Other Name   
Animal   rat Strain   Fischer
Genus   Rattus Species   norvegicus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   thymidine kinase deficient RAT-1 (F2408) cell line
Classify   mutant Established by   Topp, W. C.
Registered by   Kaisho,Y. Regulation for Distribution   
Comment    Year   1990
Medium   Dulbecco's modified Eagle's medium with 5% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0513
Reference
Pubmed id:7269249Normal rat cell lines deficient in nuclear thymidine kinase.
Topp WC
Virology. 1981 Aug;113(1):408-11

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50282 Cell Name   RAT-2
LOT No.   1005 Lot Specification   distribution
Medium   D.MEM, 95% ; FBS, 5% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   7 x 10^5
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   Unknown (8 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   アンプル印字がかすれて消えている場合があります（RA_-2）が、正しいアンプルです。
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