IFO50269 GH1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50269 Cell Name   GH1
Profile   pituitary tumor, somatotrophin-secreting, (Wistar/Furth rat) Other Name   
Animal   rat Strain   Wistar-Furth
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   pituitary
Case history   pituitary tumor Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   pituitary tumor, somatotrophin-secreting
Classify   tumor Established by   Yasumura, Y. et al.
Registered by   Igarashi,K. Regulation for Distribution   
Comment    Year   1990
Medium   Ham's F10 medium with 15% horse serum and 2.5% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   pituitary gland
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0610
Reference
Pubmed id:4951281Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone.
Tashjian AH Jr,Yasumura Y,Levine L,Sato GH,Parker ML
Endocrinology. 1968 Feb;82(2):342-52
Pubmed id:4288399Establishment of four functional, clonal strains of animal cells in culture.
Yasamura Y,Tashjian AH Jr,Sato GH
Science. 1966 Dec 2;154(3753):1186-9

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50269 Cell Name   GH1
LOT No.   1158 Lot Specification   distribution
Medium   D.MEM, 85% ; horse serum, 15% Temperature   37 C
Cell Density at Seeding   4 - 8 x 10^4 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   P61 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, AST, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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