IFO50268 EHS

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50268 Cell Name   EHS
Profile   This cell line secretes basement membrane matrix including laminin and type IV collagen. Other Name   
Animal   mouse Strain   ST/Eh
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   sarcoma
Case history   chondrosarcoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   chondrosarcoma, secreting basement membrane matrix
Classify   tumor Established by   Swarm, R. L.
Registered by   Kanamaru,T. Regulation for Distribution   
Comment    Year   1990
Medium   not cultivable in vitro Methods for Passages   in vivo, intramuscular (or subcutaneous) transplantation in C57BL/J mouse
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   spontaneous tumor
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3506
Reference
Pubmed id:14069835TRANSPLANTATION OF A MURINE CHONDROSARCOMA IN MICE OF DIFFERENT INBRED STRAINS.
SWARM RL
J Natl Cancer Inst. 1963 Oct;31():953-75

Images
PDF
   PDF File1



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50268 Cell Name   EHS
LOT No.   07282003 Lot Specification   distribution
Medium   Not cultivable. Subcutaneous transplantation (host; 10 week C57BL/6 mice). Temperature   
Cell Density at Seeding    Methods for Passages   Subcutaneous transplantation (neck). Tumors were removed from animals under sterile condition after 28 days.
Doubling Time    Cell Number in Vial (cells/1ml)   > 10^7 cells
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS in 1:1 mixture of EMEM and Hanks' BSS. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50268 Cell Name   EHS
LOT No.   374 Lot Specification   seed/distribution
Medium   in vivo transplantation in C57BL/J mouse Temperature   
Cell Density at Seeding    Methods for Passages   in vivo transplantation in C57BL/J mouse
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% fetal bovine serum - Eagle's MEM CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50268 Cell Name   EHS
LOT No.   06302021 Lot Specification   distribution
Medium   Not cultivable. Subcutaneous transplantation (host; 10 week C57BL/6 mice). Temperature   
Cell Density at Seeding    Methods for Passages   Subcutaneous transplantation (neck). Tumors were removed from animals under sterile condition. (Tumor growth will take about 4 weeks.)
Doubling Time    Cell Number in Vial (cells/1ml)   uncountable
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    10% DMSO, 20% FBS in 1:1 mixture of EMEM and Hanks' BSS. CO2 Conc.   
Viability immediately after thawing (%)   uncountable Additional information   
Images
Cell No.   IFO50268 Cell Name   EHS
LOT No.   07192021 Lot Specification   distribution
Medium   Not cultivable. Subcutaneous transplantation (host; 10 week C57BL/6 mice). Temperature   
Cell Density at Seeding    Methods for Passages   Subcutaneous transplantation (neck). Tumors were removed from animals under sterile condition. (Tumor growth will take about 4 weeks.)
Doubling Time    Cell Number in Vial (cells/1ml)   uncountable
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   MEM with 20% FBS and10% DMSO CO2 Conc.   
Viability immediately after thawing (%)   uncountable Additional information   Type of ampoules is plastic.
Images
Cell No.   IFO50268 Cell Name   EHS
LOT No.   10202022 Lot Specification   distribution
Medium   Not cultivable. Subcutaneous transplantation (host; 10 week C57BL/6 mice). Temperature   
Cell Density at Seeding   uncountable Methods for Passages   in vivo transplantation in C57BL/J mouse. Subcutaneous transplantation (neck). Tumors were removed from animals under sterile condition. (Tumor growth will take about 4 weeks.)
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   MEM+20%FBS+10%DMSO CO2 Conc.   
Viability immediately after thawing (%)   uncountable Additional information   in vivo transplantation in C57BL/J mouse Type of ampoules is plastic.
Cell No.   IFO50268 Cell Name   EHS
LOT No.   10272022 Lot Specification   distribution
Medium   Not cultivable. Subcutaneous transplantation in C57BL/J mouse). Temperature   
Cell Density at Seeding   uncountable Methods for Passages   in vivo transplantation in C57BL/J mouse. Subcutaneous transplantation (neck). Tumors were removed from animals under sterile condition. (Tumor growth will take about 4 weeks.)
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   MEM+20%FBS+10%DMSO CO2 Conc.   
Viability immediately after thawing (%)   uncountable Additional information   in vivo transplantation in C57BL/J mouse
CLOSE