IFO50267 PB-3c

Cell information

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IFO50267 PB-3cの培養

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50267 Cell Name   PB-3c
Profile   IL-3-dependent mouse bone marrow cell line. Other Name   
Animal   mouse Strain   
Genus   Mus Species   musculus
Sex    Age   
Identity   not done Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   mast cell line
Classify   tumor Established by   Ball, P. E.
Registered by   Shimizu,T. Regulation for Distribution   
Comment    Year   1990
Medium   RPMI1640 with 10% fetal bovine serum, 10% WEHI-3b conditioned medium and 50 uM 2-mercaptoethanol Methods for Passages   Simple dilution (suspension culture). Since the size of this cell line is very small, centrifuge at 1200 - 1500 rpm x 5 min and ascertain that the cells are fully sedimented when you collect the cells.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5346
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50267 Cell Name   PB-3c
LOT No.   333 Lot Specification   seed/distribution
Medium   RPMI 1640, 80% ; FBS, 10% ; WEHI conditioned medi um, 10% ; 0.05mM 2-mercaptoethanol Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (1 at IFO) PDL   
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50267 Cell Name   PB-3c
LOT No.   10042013 Lot Specification   distribution
Medium   RPMI1640 with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099, Lot 632877), 10% WEHI-3b conditioned medium and 50 uM 2-mercaptoethanol Temperature   37C
Cell Density at Seeding   1.2 - 1.5 x 10^5 cells/mL Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined. Mouse confirmed.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   90.1 Additional information   
Cell No.   IFO50267 Cell Name   PB-3c
LOT No.   02172025 Lot Specification   distribution
Medium   RPMI 1640 medim with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K), 10% WEHI-3b conditioned medium and 50 uM 2-mercaptoethanol Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time    Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   85.9 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   72.9 Additional information   
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