IFO50099 F2408-B812

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50099 Cell Name   F2408-B812
Profile   HGPRT-deficient line from F2408 (IFO 50015). This cell line exhibits temperature sensitive phenotype for transformation by Moloney MSV. Other Name   
Animal   rat Strain   Fischer
Genus   Rattus Species   norvegicus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   HGPRT-deficient. Temperature sensitive when this cell line is transformed by MSV.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   HGPRT-deficient cell line from F2408. This cell line exhibits temperature sensitive phenotype for transformation by Moloney MSV. The transforming efficiency considerably decreases at 39 C compared to at 33 C.
Classify   mutant Established by   Inoue, H.
Registered by   Hakura,A. Regulation for Distribution   
Comment    Year   1986
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryonic fibroblast
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_E951
Reference
Pubmed id:6829165Rat mutant cells showing temperature sensitivity for transformation by wild-type Moloney murine sarcoma virus.
Inoue H,Yutsudo M,Hakura A
Virology. 1983 Feb;125(1):242-5

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50099 Cell Name   F2408-B812
LOT No.   1018 Lot Specification   distribution
Medium   D.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   9 x 10^5
Viability at cell freezing (%)   73 Antibiotics Used   free
Passage Number   Unknown (>3 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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