IFO50080 NCTC clone 1469

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50080 Cell Name   NCTC clone 1469
Profile   The NCTC clone 1469 is a subline of NCTC 721. Other Name   
Animal   mouse Strain   C3H/AN
Genus   Mus Species   musculus
Sex   M Age   48-hour-old
Identity    Tissue for Primary Cancer   liver
Case history   normal Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   normal liver-derived
Classify    Established by   Hobbs, G. L. et al.
Registered by   Inoue,M. Regulation for Distribution   
Comment    Year   1985
Medium   NCTC 135 medium with 10% horse serum Methods for Passages   scraping, subcultivation ratio 1/2 - 1/4
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3066
Reference
Pubmed id:14006338Characterization of cells in tissue culture.
WESTFALL BB
Natl Cancer Inst Monogr. 1962 Apr;7():147-58
Pubmed id:13766009A hepatitis virus complicating studies with mouse leukemia.
MANAKER RA,PICZAK CV,MILLER AA,STANTON MF
J Natl Cancer Inst. 1961 Jul;27():29-51
Pubmed id:14431674Glycogen content of cell suspensions prepared from massive tissue culture. II. Comparison of fourteen cell strains after long cultivation.
PEPPERS EV,WESTFALL BB,EARLE WR
J Natl Cancer Inst. 1959 Oct;23():823-31
Pubmed id:13619759Long-term cultivation of animal tissue cells in large cultures.
EARLE WR
Fed Proc. 1958 Dec;17(4):967-74
Pubmed id:13523589Studies on culture lines derived from mouse liver parenchymatous cells grown in long-term tissue culture.
EVANS VJ,HAWKINS NM,WESTFALL BB,EARLE WR
Cancer Res. 1958 Apr;18(3):261-6
Pubmed id:13502690Establishment of a clone of mouse liver cells from a single isolated cell.
HOBBS GL,SANFORD KK,EVANS VJ,EARLE WR
J Natl Cancer Inst. 1957 May;18(5):701-7
Pubmed id:14939026The growth in vitro of massive cultures of liver cells.
EVANS VJ,EARLE WR,WILSON EP,WALTZ HK,MACKEY CJ
J Natl Cancer Inst. 1952 Jun;12(6):1245-65

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50080 Cell Name   NCTC clone 1469
LOT No.   413 Lot Specification   distribution
Medium   NCTC 135, 90% and horse serum, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   scraping, subcultivation ratio = 1/2 - 1/4
Doubling Time    Cell Number in Vial (cells/1ml)   7 x 10^6
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   P730 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   Labeled as "NCTC1469, R217_F413".
Cell No.   IFO50080 Cell Name   NCTC clone 1469
LOT No.   01282021 Lot Specification   distribution
Medium   NCTC 135 medium with 10% horse serum Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^5 cells/mL Methods for Passages   Scraping, split ratio 1/2 - 1/4.
Doubling Time   approx. 41 hrs. Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   P731 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% HS - NCTC 135 CO2 Conc.   5%
Viability immediately after thawing (%)   89.9 Additional information   
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