IFO50079 Flow7000
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50079 | Cell Name | Flow7000 |
|---|---|---|---|
| Profile | Normal diploid fibroblasts with limited life span. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | 14-week fetus | |
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | skin normal diploid fibroblast |
| Classify | primary | Established by | |
| Registered by | Sasada,R. | Regulation for Distribution | |
| Comment | Year | 1985 | |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA, subcultivation ratio =1/2 |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | prepuce |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | tested | ||||||
| HCV | - | HTLV-1 | - | ||||||
| HIV-1 | - | HTLV-2 | - | ||||||
| HIV-2 | - | HAV | - | ||||||
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-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:841332 | Enzyme polymorphisms as genetic signatures in human cell cultures. O'Brien SU,Kleiner G,Olson R,Shannon JE Science. 1977 Mar 25;195(4284):1345-8 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | IFO50079 | Cell Name | |
|---|---|---|---|
| LOT No. | 04092004 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum ( FBS lot; GIBCO 3762782S) | Temperature | 37 C |
| Cell Density at Seeding | 2-3 x 10^4 cells/sq.cm | Methods for Passages | 0.25% trypsin and 0.02% EDTA. This lot of cells can grow at least additional 3 passages at 1/4 split. |
| Doubling Time | ca. 3 days | Cell Number in Vial (cells/1ml) | 7.5 x 10^5 |
| Viability at cell freezing (%) | 93.8 | Antibiotics Used | free |
| Passage Number | 9 at bank | PDL | +10.4 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type A), AST and LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50079 | Cell Name | Flow7000 |
|---|---|---|---|
| LOT No. | 335 | Lot Specification | distribution |
| Medium | E.MEM, 90% + FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA, subcultivation ratio ; 1:2 | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 2 x 10^6 | |
| Viability at cell freezing (%) | 65 | Antibiotics Used | free |
| Passage Number | Unknown (5 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NP, MD, LD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:9,13 D13S317:12 D7S820:9,12 D16S539:10,12 VWA:15,17 TH01:8 AM:X TPOX:8,11 CSF1PO:9,11 |
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| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | IFO50079 | Cell Name | Flow7000 |
|---|---|---|---|
| LOT No. | 10132017 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 0.3 - 1.1 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 42 hrs. | Cell Number in Vial (cells/1ml) | 6.4 x 10^5 |
| Viability at cell freezing (%) | 89.3 | Antibiotics Used | free |
| Passage Number | Unknown (9 at bank) | PDL | +11 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 89.3 | Additional information |