IFO50076 IT-45R1

Cell information

Important Notice(s)

Dispaseの単位について

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50076 Cell Name   IT-45R1
Profile   T cell differentiation factor producing, (Wistar rat) Other Name   
Animal   rat Strain   Wistar
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   thymus
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   T cell differentiation factor-producing
Classify    Established by   Itoh, T.
Registered by   Itoh,T. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   dispase (500 units/ml) in culture medium.
Cell Number on Passage   Subcultivation ratio=1/3. Race   
CO2 Conc.   5 % Tissue Sampling   thymus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_7677
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50076 Cell Name   IT-45R1
LOT No.   975 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dispase (500 Units/ml), subcultivation ratio ; 1:3
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   Unknown (10 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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