IFO50074 HFL1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50074 | Cell Name | HFL1 |
|---|---|---|---|
| Profile | Normal diploid fibroblasts with limited life span. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | 16-18 week | |
| Identity | available | Tissue for Primary Cancer | lung |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | finite | Crisis PDL | 57 |
| Morphology | fibroblast-like | Character | fetus lung normal diploid fibroblast |
| Classify | primary | Established by | Bradley, K. |
| Registered by | Takeuchi,M. | Regulation for Distribution | |
| Comment | Year | 1985 | |
| Medium | Ham's F12K medium with 15% fetal bovine serum | Methods for Passages | trypsin |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | lung |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | tested | ||||||
| HCV | - | HTLV-1 | - | ||||||
| HIV-1 | - | HTLV-2 | - | ||||||
| HIV-2 | - | HAV | - | ||||||
|
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:7372635 | Control of collagen production by human diploid lung fibroblasts. Breul SD,Bradley KH,Hance AJ,Schafer MP,Berg RA,Crystal RG J Biol Chem. 1980 Jun 10;255(11):5250-60 |
| Images |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | IFO50074 | Cell Name | HFL1 |
|---|---|---|---|
| LOT No. | 1168 | Lot Specification | distribution |
| Medium | Ham's F12K, 85% ; FBS, 15% | Temperature | 37 C |
| Cell Density at Seeding | 3 x 10^4 cells/ml | Methods for Passages | trypsin-EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 6.6 x 10^5 | |
| Viability at cell freezing (%) | 91 | Antibiotics Used | free |
| Passage Number | P16 | PDL | +6 |
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | NP, G6PD (type B), LD | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.)-culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | IFO50074 | Cell Name | HFL1 |
|---|---|---|---|
| LOT No. | 10092015 | Lot Specification | distribution |
| Medium | Ham's F12K medium with 15% fetal bovine serum (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | approx. 4 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 31 hrs. | Cell Number in Vial (cells/1ml) | 1.0 x 10^6 |
| Viability at cell freezing (%) | 83 | Antibiotics Used | free |
| Passage Number | P17 | PDL | unknown |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - Ham's F12K | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 83 | Additional information |