IFO50073 MRC-5

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50073 Cell Name   MRC-5
Profile   Normal diploid fibroblasts with limited life span (crisis PDL = 42-46). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   14-week fetus
Identity   available Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   42-46
Morphology   fibroblast-like Character   fetus lung normal diploid fibroblast
Classify   primary Established by   Jacobs, J. P.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1985
Medium   Basal medium Eagle (Hanks' salt) with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0440
Reference
Pubmed id:7389832A new human diploid cell strain, TIG-1, for the research on cellular aging.
Ohashi M,Aizawa S,Ooka H,Ohsawa T,Kaji K,Kondo H,Kobayashi T,Noumura T,Matsuo M,Mitsui Y,Murota S,Yamamoto K,Ito H,Shimada H,Utakoji T
Exp Gerontol. 1980;15(2):121-33
Pubmed id:12635218Error theory and ageing in human diploid fibroblasts.
Lewis CM,Tarrant GM
Nature. 1972 Oct 6;239(5371):316-8
Pubmed id:12635262Altered enzymes in ageing human fibroblasts.
Holliday R,Tarrant GM
Nature. 1972 Jul 7;238(5358):26-30
Pubmed id:4316953Characteristics of a human diploid cell designated MRC-5.
Jacobs JP,Jones CM,Baille JP
Nature. 1970 Jul 11;227(5254):168-70

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50073 Cell Name   
LOT No.   08072009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   5 - 7 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Split ratio 1/4 - 1/6.
Doubling Time   45 hrs Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   88.2 Antibiotics Used   free
Passage Number   P24 PDL   33
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50073 Cell Name   MRC-5
LOT No.   1094 Lot Specification   distribution
Medium   Eagle's Basal Medium in Hanks' BSS, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   7 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P25 PDL   34
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD (type B), MD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50073 Cell Name   MRC-5
LOT No.   02272015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.6 - 1.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 40 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   72 Antibiotics Used   free
Passage Number   P24 PDL   34
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   72 Additional information   
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