IFO50043 WiDr
Cell information
Important Notice(s)JCRB0224, IFO50043 WiDr is derivative from HT-29.
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50043 | Cell Name | WiDr |
|---|---|---|---|
| Profile | adenocarcinoma | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 78 |
| Identity | available | Tissue for Primary Cancer | colon |
| Case history | adenocarcinoma | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | adenocarcinoma |
| Classify | tumor | Established by | Wallace, R. |
| Registered by | Suzuki,N. | Regulation for Distribution | |
| Comment | WiDr cell line is suggested to be derived from a human colon adenocarcinoma cell line HT-29 (Cancer Genet. Cytogenet., 27,125 (1987)) | Year | 1985 |
| Medium | Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | colon |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | tested | ||||||
| HCV | - | HTLV-1 | - | ||||||
| HIV-1 | - | HTLV-2 | - | ||||||
| HIV-2 | - | HAV | - | ||||||
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-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | IFO50043 | Cell Name | WiDr |
|---|---|---|---|
| LOT No. | 1160 | Lot Specification | distribution |
| Medium | E.MEM with non-essential amino acids, 90%; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | 1-1.5 x 10^5 cells/ml | Methods for Passages | trypsin-EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 4.5 x 10^6 | |
| Viability at cell freezing (%) | 94 | Antibiotics Used | free |
| Passage Number | Unknown (6 at IFO) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD, AST, LD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:11,12 D13S317:11,12 D7S820:10 D16S539:11,12 VWA:17,19 TH01:6,9 AM:X TPOX:8,9 CSF1PO:11,12 |
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| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.)-culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |