IFO50036 P388D1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50036 Cell Name   P388D1
Profile   lymphoid neoplasm, derived from P388, (DBA/2 mouse) Other Name   
Animal   mouse Strain   DBA/2
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   lymphoid neoplasm Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   lymphoid neoplasm, derived from P388
Classify   tumor Established by   Dawe, C. J. & Potter, M.
Registered by   Iwasa,S. Regulation for Distribution   
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   lymph node
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0477
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50036 Cell Name   P388D1
LOT No.   337 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (5 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, MD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50036 Cell Name   P388D1
LOT No.   338 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (5 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, MD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50036 Cell Name   P388D1
LOT No.   04102019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.3 - 2.6 x 10^5 cells/mL Methods for Passages   Simple dilution.
Doubling Time   approx. 25 hrs. Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   94.5 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   82.8 Additional information   
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