IFO50025 IM-9

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50025 Cell Name   IM-9
Profile   IgG-secreting B-lymphoblastic cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   multiple myeloma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   round Character   B-lymphoblastoid cells, EB virus-transformed
Classify   tumor Established by   Buell, D. N.
Registered by   Yoshida,T. Regulation for Distribution   
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1305
Reference
Pubmed id:4359334Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture.
Gavin JR 3rd,Roth J,Neville DM Jr,de Meyts P,Buell DN
Proc Natl Acad Sci U S A. 1974 Jan;71(1):84-8

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50025 Cell Name   IM-9
LOT No.   1151 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   70 Antibiotics Used   free
Passage Number   Unknown (5 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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