IFO50020 SIRC

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50020 Cell Name   SIRC
Profile   susceptible to rubella virus Other Name   
Animal   rabbit Strain   
Genus   Orycytologus Species   cuniculus
Sex    Age   adult
Identity    Tissue for Primary Cancer   cornea
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   susceptible to rubella virus
Classify    Established by   Volkert, M.
Registered by   Hakura,A. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   cornea
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2724
Reference
Pubmed id:6042441Plaque assays of rubella virus in cultures of rabbit cornea (SIRC) cells.
Rhim JS,Schell K,Huebner RJ
Proc Soc Exp Biol Med. 1967 Aug-Sep;125(4):1271-4
Pubmed id:5918951Isolation, propagation and neutralization of rubella virus in cultures of rabbit cornea (SIRC) cells.
Phillips CA,Melnick JL,Burkhardt M
Proc Soc Exp Biol Med. 1966 Jul;122(3):783-6

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50020 Cell Name   SIRC
LOT No.   1161 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   90.1 Antibiotics Used   free
Passage Number   Unknown (>3 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, AST, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   Ampoule mislabeling: IFO50022 is error, correctly IFO50020.
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