IFO50018 NIL

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50018 Cell Name   NIL
Profile   transformed from hamster fibroblast Other Name   
Animal   hamster, Syrian Strain   Syrian
Genus   Mesocricetus Species   auratus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   transformed from hamster fibroblast
Classify   transformed Established by   Diamond, L.
Registered by   Hakura,A. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryo, whole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8738
Reference
Pubmed id:1168186Surface carbohydrates of hamster fibroblasts. II. Interaction of hamster NIL cell surfaces with Ricinus communis lectin and concanavalin A as revealed by surface galactosyl label.
Gahmberg CG,Hakomori S
J Biol Chem. 1975 Apr 10;250(7):2447-51
Pubmed id:164440Surface carbohydrates of hamster fibroblasts. I. Chemical characterization of surface-labeled glycosphingolipids and aspecific ceramide tetrasaccharide for transformants.
Gahmberg CG,Hakomori S
J Biol Chem. 1975 Apr 10;250(7):2438-46
Pubmed id:4346327Glycolipid synthesis and tumorigenicity of clones isolated from the Nil 2 line of hamster embryo fibroblasts.
Sakiyama H,Robbins PW
Fed Proc. 1973 Jan;32(1):86-90
Pubmed id:6039764Two spontaneously transformed cell lines derived from the same hamster embryo culture.
Diamond L
Int J Cancer. 1967 Mar 15;2(2):143-52

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50018 Cell Name   NIL
LOT No.   348 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   9 x 10^5
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50018 Cell Name   NIL
LOT No.   349 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   10 x 10^5
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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