IFO50005 J-111

Cell information

Important Notice(s)

JCRB0073, IFO50005 J-111 is HeLa-derivative / H-111細胞のHeLa細胞汚染

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50005 Cell Name   J-111
Profile   HeLa markers* Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   HeLa-contaminant
Case history    Metastasis   
Tissue Metastasized    Genetics   HeLa markers observed.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   originally established from acute monocytic leukemia, HeLa-contaminated
Classify   tumor Established by   Osgood, E. E. & Brooke, J. H.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment   *Contamination of HeLa cells has been suspected in this line. This cell line is different from the CRL-8129 in ATCC although the name is same. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2965
Reference
Pubmed id:14185313ANIMAL CELL STRAINS. THE CELL CULTURE COLLECTION COMMITTEE HAS ASSEMBLED AND CERTIFIED 23 STRAINS OF ANIMAL CELLS.
LEDLEY RS
Science. 1964 Oct 9;146(3641):241-3
Pubmed id:13260360Continuous tissue culture of leukocytes from human leukemic bloods by application of gradient principles.
OSGOOD EE,BROOKE JH
Blood. 1955 Oct;10(10):1010-22

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50005 Cell Name   J-111
LOT No.   74 Lot Specification   distribution
Medium   E.MEM, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   4 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (>6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, MD, MPI
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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