JCRB0157 TK

Cell information

Important Notice(s)

Media for JCRB0157 TK, JCRB0158.0-0158.2 MY**, JCRB0159 SLVL

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0157 Cell Name   TK
Profile   Human B-cell lymphoma cell line with near-triploid chromosomes with t(14;18) and p53 gene point mutation. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   80
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   B-cell malignant lymphoma. Metastasis   
Tissue Metastasized    Genetics   CD10, CD19, CD20, HLA-DR positive. A codon 180 of the p53 mutated (GAG -> GAT). Near-triploid chromosomes with t(14;18)(q32;q21), add(17)(p22).
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   A rare large B-cell non-Hodgkin's lymphoma having a near-triploid cell population.
Classify   tumor Established by   Inokuchi,K. et.al.
Registered by   Inokuchi,K. Regulation for Distribution   not defined
Comment    Year   2000
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3216
Reference
Pubmed id:10354143Establishment of a near-triploid human B-cell lymphoma cell line with t(14;18) and a p53 gene point mutation.
Yamaguchi H,Inokuchi K,Hanawa H,Tanosaki S,Shinozawa I,Matuoka H,Dan K
Br J Haematol. 1999 Jun;105(3):764-7

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0157 Cell Name   TK
LOT No.   08032007 Lot Specification   distribution
Medium   alpha-MEM with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   Dilution. This cell line was weak for freezing, therefore over half of cells will die after thawing.
Doubling Time   30 - 40 hrs Cell Number in Vial (cells/1ml)   6.0 x 10^6 (total)
Viability at cell freezing (%)   73 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - alpha MEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0157 Cell Name   TK
LOT No.   01302001 Lot Specification   distribution
Medium   alpha-MEM with 10% heat inactivated fetal calf serum (Intergen RB2405). Temperature   37 C
Cell Density at Seeding   1.0 x 10^5 Methods for Passages   Subculture was done by simple dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   92.8 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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