JCRB0154 NB(TU)1-10

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0154 Cell Name   NB(TU)1-10
Profile   Humn neuroblastoma(stageIII) cell line from abdominal tumor with N-myc aberration. Other Name   NB(TU)1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   20-month-old
Identity   available Tissue for Primary Cancer   neural
Case history   abdominal tumor. Tumor(6cm) found adjacent to the right lobe of liver. Stage III neuroblastoma. Metastasis   
Tissue Metastasized    Genetics   N-myc aberration observed. Genetically instable cell line. Pseudo-diploid.
Life Span   infinite Crisis PDL   
Morphology    Character   Neuroblastoma cell line with genetically instable feature established from stage III case of tumor.
Classify   tumor Established by   Inoue,A. & Shimatake,H.
Registered by   Inoue,A. Regulation for Distribution   
Comment   Cells will be available in near future. Year   1996
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum. Methods for Passages   Cells dispersed by pipetting.
Cell Number on Passage   5.5 x 10^3 cells/sq.cm. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   adrenal grand
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3041
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1240181
Reference
Pubmed id:9378542Extensive genetic heterogeneity in the neuroblastoma cell line NB(TU)1.
Inoue A,Yokomori K,Tanabe H,Mizusawa H,Sofuni T,Hayashi Y,Tsuchida Y,Shimatake H
Int J Cancer. 1997 Sep 17;72(6):1070-7

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0154 Cell Name   NB(TU)1-10
LOT No.   10252000 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal calf serum (Intergen RB2405). Temperature   37 C
Cell Density at Seeding   1.7 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested with pipetting and then diluted.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   80.5 Antibiotics Used   free
Passage Number   P10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(human type B), NP, LDH examined. Human.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:12
D7S820:12
D16S539:9,12
VWA:14
TH01:9
AM:X
TPOX:11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB0154 Cell Name   NB(TU)1-10
LOT No.   06102019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1:4 split Methods for Passages   Cells were harvested by pipetting, and diluted to fresh medium.
Doubling Time   4 folds increase/week Cell Number in Vial (cells/1ml)   Uncountable (aggregates)
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   Unknown (13 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   Uncountable Additional information   
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