JCRB0132.1 AH601(JTC27)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0132.1 Cell Name   AH601(JTC27)
Profile   A transplantable rat hepatoma cell line derived from ascites. Other Name   JTC27
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   liver
Case history   hepatoma, Hepatoma induced by feeding aminoazotoluene. Metastasis   
Tissue Metastasized    Genetics   hepatoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   transplantable rat ascites tumor cell line. Rat established on 1952 and tumor was maintained by transplantation. Primary cell culture was established on 1971. Serum free cell line was established on 1972.
Classify   tumor Established by   Takaoka,T. & Katsuta,H.
Registered by   Takaoka,T. Regulation for Distribution   free
Comment    Year   2000
Medium   DM201 medium with 2 % fetal bovine serum. Add fetal bovine serum at the concentration of 10% for initial cell attachment after thawing (one over night). Methods for Passages   Cells harvested after treatment with 0.25% trypsin.
Cell Number on Passage   Split ratio = 1/8. Race   
CO2 Conc.   5 % Tissue Sampling   ascites
Tissue Type   hepatoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2085
Reference
Pubmed id:4340679Tissue culture of rat ascites hepatoma AH-601 cells.
Huh N,Takaoka T,Katsuta H
Jpn J Exp Med. 1972 Jun;42(3):249-62

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0132.1 Cell Name   AH601(JTC27)
LOT No.   06232000 Lot Specification   distribution
Medium   DM201 medium with 2% fetal calf serum (Intergen RB2405). Temperature   37 C
Cell Density at Seeding   1-4 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin in 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   99.3 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LDH, G6PD, NP. Rat.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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