JCRB0144.1 LYM-1.P3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0144.1 Cell Name   LYM-1.P3
Profile   The cells are cultured under serum free condition that are derived from lymph node. Cells are sticky to culture dishes. Other Name   
Animal   rat Strain   JAR-2
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   lymph node
Case history   normal Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Sticky cell from lymph node. Serum free cell culture.
Classify   serum free Established by   Kurayama,H.
Registered by   Takaoka,T. Regulation for Distribution   free
Comment    Year   1999
Medium   DM201 medium with serum free condition. Methods for Passages   Cells harvested by gentle scraping.
Cell Number on Passage   1.3 x 10^4 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   lymph node
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_U213
Reference
Pubmed id:9511820The value of LYM-1 cells for examining vacuole formation and loss of cell viability induced by culture supernates of Helicobacter pylori.
Wang XM,Kojima T,Satoh K,Taniguchi Y,Tokumaru K,Saifuku K,Seki M,Kihira K,Ido K,Uchida JY,Ohmori C,Takaoka T,Kimura K
J Med Microbiol. 1997 Aug;46(8):705-9

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0144.1 Cell Name   LYM-1.P3
LOT No.   02012000 Lot Specification   distribution
Medium   DM201 medium without serum. Serum free cell culture. Temperature   37 C
Cell Density at Seeding   2.5 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested by scraper and resuspended in fresh medium. Subculture every 4-5 days and change medium every 2 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, NP and LDH examined. Rat.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   A 1 to 1 mixture of the culture medium and FM-1 medium containing 10% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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