JCRB0536 TIG-121

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0536 Cell Name   TIG-121
Profile   skin, normal diploid fibroblast Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   8-month-old
Identity   available Tissue for Primary Cancer   skin (leg)
Case history   primary, normal fibroblast Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   normal diploid
Classify    Established by   
Registered by   Tokyo Metropolitan I Regulation for Distribution   
Comment   In the paper referring this cell line (Exp. Cell Res. 220: 501 (1995)), the cell name was mistyped as TIG-120 (in Material & Method section). Year   1999
Medium   Eagle's minimum essential medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3203
Reference
Pubmed id:7556460Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration.
Kondo H,Yonezawa Y
Exp Cell Res. 1995 Oct;220(2):501-4




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0536 Cell Name   
LOT No.   09272010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   approx. 1.5 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 42 hrs. Cell Number in Vial (cells/1ml)   4.6 x 10^5
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   18
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0536 Cell Name   
LOT No.   R211 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% FBS This lot of ampule was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   split ratio = 1:4 Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   90.7 Antibiotics Used   100 units/ml penicillin and 100 ug/ml streptomysin
Passage Number    PDL   18
Sterility: MYCOPLASMA    Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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