JCRB0137 RERF-LC-KJ
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0137 | Cell Name | RERF-LC-KJ |
|---|---|---|---|
| Profile | adenocarcinoma, transplantable to SCID mice and frequently metastasize to lymph nodes | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 78 |
| Identity | available | Tissue for Primary Cancer | lung (cancer) |
| Case history | lung cancer | Metastasis | No |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | NT |
| Morphology | epithelial-like | Character | Transplantable to SCID mice and frequently metastasize to SCID mouse lymph nodes. |
| Classify | tumor | Established by | Teraoka,S. & Kyoizumi,S. et al |
| Registered by | Kyoizumi,S. | Regulation for Distribution | not specified |
| Comment | Year | 1999 | |
| Medium | RPMI1640 medium with 10% heat inactivated fetal calf serum. | Methods for Passages | Harvest cells with 0.125% trypsin and 0.01% EDTA at 37 C for 5 minutes. Subculture once a week at split ratio=1/4. |
| Cell Number on Passage | Split ratio=1/4. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | lung |
| Tissue Type | adenocarcinoma, T4N3M1 |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:7790314 | A novel SCID mouse model for studying spontaneous metastasis of human lung cancer to human tissue. Teraoka S,Kyoizumi S,Seyama T,Yamakido M,Akiyama M Jpn J Cancer Res. 1995 May;86(5):419-23 |
| Pubmed id:21559605 | Scid mice model for the in-vivo study of human oncotherapy - studies on the growth and metastasis of human lung-cancer. Teraoka S,Kyoizumi S,Seyama T,Yamakido M,Akiyama M Int J Oncol. 1994 Sep;5(3):501-8 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0137 | Cell Name | RERF-LC-KJ |
|---|---|---|---|
| LOT No. | 09132010 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 2x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA and split (1/4-1/6) once a week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.36x10^6 |
| Viability at cell freezing (%) | 99.2 | Antibiotics Used | free |
| Passage Number | p21 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10 D13S317:10 D7S820:10,11 D16S539:11 VWA:17 TH01:7,9 AM:X,Y TPOX:11 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0137 | Cell Name | |
|---|---|---|---|
| LOT No. | 092499 | Lot Specification | distribution |
| Medium | RPMI1640 medium with heat-inactivated fetal bovine serum (BioWhittaker 6M0121). | Temperature | 37 C |
| Cell Density at Seeding | 1-2 x 10^5 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | D.T.=ca 56 hrs | Cell Number in Vial (cells/1ml) | 8.1 x 10^5 |
| Viability at cell freezing (%) | 86 | Antibiotics Used | free |
| Passage Number | P23 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0137 | Cell Name | RERF-LC-KJ |
|---|---|---|---|
| LOT No. | 06252013 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat inactivated fetal calf serum (FBS; GIBCO Cat. # 10099). | Temperature | 37C |
| Cell Density at Seeding | Split ratio=1/4. | Methods for Passages | Harvest cells with 0.25% trypsin and 0.02% EDTA at 37 C for 5 minutes. Subculture once a week at split ratio=1/4. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1.4 x 10^6 (cells/1 | |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | P21 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB),NP,LDH were examined. Human,not HeLa |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | 5% | |
| Viability immediately after thawing (%) | 77.6 | Additional information |