JCRB0135 MT-4
Cell information
Cell type：virus production cells (View Pricing Information)
| JCRB No. | JCRB0135 | Cell Name | MT-4 |
|---|---|---|---|
| Profile | HTLV-I-transformed leukocytes, HTLV-I antigen-positive. (This cell line is supplied from National Institute of Biomedical Innovation.) | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | |
| Classify | tumor | Established by | |
| Registered by | Regulation for Distribution | ||
| Comment | Year | 1992 | |
| Medium | Methods for Passages | ||
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | + | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | NT | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:11336546 | Induction of Tax i expression in MT-4 cells by 5-azacytidine leads to protein binding in the HTLV-1 LTR in vivo. Datta S,Kothari NH,Fan H Virology. 2001 May 10;283(2):207-14 |
| Pubmed id:8927062 | [Cloning proviral DNA sequences of the human T-cell leukemia virus (HTLV-1) from the genome of cultured MT-4 cells]. Susloparov MA,Krendel'shchikov AV,Bakhtina MM,Bedristov AI,Babkin IV,Gutorov VV,Eroshkin AM,Mamaeva OA Mol Gen Mikrobiol Virusol. 1996 Apr-Jun;(2):32-9 |
| Pubmed id:7988730 | Autoantigen Ku protein is involved in DNA binding proteins which recognize the U5 repressive element of human T-cell leukemia virus type I long terminal repeat. Okumura K,Takagi S,Sakaguchi G,Naito K,Minoura-Tada N,Kobayashi H,Mimori T,Hinuma Y,Igarashi H FEBS Lett. 1994 Dec 12;356(1):94-100 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0135 | Cell Name | |
|---|---|---|---|
| LOT No. | 102799 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% triptose phosphate broth and 10% fetal bovine serum (BioWhittaker 6M0122) | Temperature | 37 C |
| Cell Density at Seeding | 1.3 x 10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | D.T. = ca 30 hrs | Cell Number in Vial (cells/1ml) | 1.4 x 10^6 |
| Viability at cell freezing (%) | 90 | Antibiotics Used | free |
| Passage Number | Unknown (5 at cellbank) | PDL | |
| Sterility: MYCOPLASMA | NT | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | Culture medium contaning 10% DMSO | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |