JCRB0131 RL-33
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0131 | Cell Name | RL-33 |
|---|---|---|---|
| Profile | Japansese white rabbit lung cell line sensitive to HSV or VSV. | Other Name | |
| Animal | rabbit | Strain | Japanese white |
| Genus | Species | ||
| Sex | Age | Young adult | |
| Identity | Tissue for Primary Cancer | lung | |
| Case history | normal | Metastasis | No |
| Tissue Metastasized | Genetics | HSV,VSV sensitive.Good to study anti-viral agents. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | Cell growth inhibited by cell-cell contact. |
| Classify | immortalized | Established by | Yoshii,T. & Kono,R. |
| Registered by | Yoshii,T. | Regulation for Distribution | free |
| Comment | Year | 1998 | |
| Medium | Eagle's minimal essential medium with 7-8% fetal bovine serum or calf serum and 0.11% NaHCO3. | Methods for Passages | Culture at 37 C and harvest cells by treatment with 0.25% trypsin and 0.02% EDTA. |
| Cell Number on Passage | Split ratio=1/5 (or 8) | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | lung |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Images |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0131 | Cell Name | RL-33 |
|---|---|---|---|
| LOT No. | 100698 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serrum. | Temperature | 37 C |
| Cell Density at Seeding | 4x10^4 cells/sq.cm. | Methods for Passages | Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.4 x 10^6 |
| Viability at cell freezing (%) | 90.3 | Antibiotics Used | free |
| Passage Number | P314 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD, LD, NP examined. Speceis confirmed. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |