JCRB0130 PC-12.P3
Cell information
Important Notice(s)Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0130 | Cell Name | PC-12.P3 |
|---|---|---|---|
| Profile | Rat pheochromo cytoma cell line derived from adrenal medulla. Use serum free DM201 media. | Other Name | PC12.P3 |
| Animal | rat | Strain | |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | neural | |
| Case history | pheochromo cytoma of the adrenal medulla. Use serum free DM201 media. | Metastasis | |
| Tissue Metastasized | Genetics | Derivative of the PC-12 cells. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Cells adapted to grow in serum free medium. |
| Classify | serum free | Established by | Takaoka,T. & Katsuta,H. |
| Registered by | Takaoka,T. | Regulation for Distribution | |
| Comment | Year | 1998 | |
| Medium | DM201 medium. Serum free (both lipid and protein free) medium used. | Methods for Passages | Harvest cells with scraper carefully. |
| Cell Number on Passage | 2 x 10^5 cells/sq.cm. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | adrenal medulla |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1493541 | Different ability in PC12 clones to recover from MMC toxicity following NGF treatment. Iizuka H,Umeda M Brain Res. 1992 Dec 18;599(1):1-5 |
| Pubmed id:3839317 | Molecular cloning of a gene sequence regulated by nerve growth factor. Levi A,Eldridge JD,Paterson BM Science. 1985 Jul 26;229(4711):393-5 |
| Pubmed id:6641712 | A macromolecular structure favouring microtubule assembly in NGF-differentiated pheochromocytoma cells (PC12). Biocca S,Cattaneo A,Calissano P EMBO J. 1983;2(5):643-8 |
| Pubmed id:742018 | [Therapeutic results in adult acute leukemia in Giessen]. Löffler H,Pralle H,Graubner M,Schrezenmaier E Verh Dtsch Ges Inn Med. 1978;(84):156-8 |
| Pubmed id:1065897 | Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Greene LA,Tischler AS Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-8 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0130 | Cell Name | PC-12.P3 |
|---|---|---|---|
| LOT No. | 060398 | Lot Specification | distribution |
| Medium | DM201 medium. Serum free (both lipid and protein free) medium used. | Temperature | 37 C |
| Cell Density at Seeding | 2 x 10^5 cells/sq.cm | Methods for Passages | Harvest cells with scraper carefully. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.1x10^6 |
| Viability at cell freezing (%) | 85.0 | Antibiotics Used | free |
| Passage Number | P4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |