JCRB0128 TK-1
Cell information
Important Notice(s)Cross-Culture contamination of TK-1 with U251 / JCRB0128 TK-1とU-251MGのクロスコンタミ
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0128 | Cell Name | TK-1 |
|---|---|---|---|
| Profile | Stop providing for the issue of quality control. (Please contact us for more information) Human glioblastoma multiforme cell line. Cross-Culture contamination with U251 suspected. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 22 |
| Identity | available | Tissue for Primary Cancer | neural |
| Case history | glioblastoma multiforme | Metastasis | No |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | NT |
| Morphology | fibroblast-like | Character | |
| Classify | tumor | Established by | Tsuboi,K. |
| Registered by | Tsuboi,K. | Regulation for Distribution | |
| Comment | STR-PCR experiments suggested the cross-culture contamination with the U251 (RCB0461). | Year | 1998 |
| Medium | Methods for Passages | ||
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | brain |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:9183640 | [Establishment of human glioblastoma cell line "TK-1"]. Tsuboi K,Yoshii Y,Nose T Hum Cell. 1996 Jun;9(2):125-8 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0128 | Cell Name | TK-1 |
|---|---|---|---|
| LOT No. | 063098 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum (lot.Rehatuin LB94203). | Temperature | 37 C |
| Cell Density at Seeding | 1.6 x 10^4 cells/sq.cm. | Methods for Passages | Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Subculture every 4-5 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.4 x 10^6 |
| Viability at cell freezing (%) | 89.0 | Antibiotics Used | free |
| Passage Number | P10* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB), LD, NP. Human. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
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