JCRB0649 HeLa.P3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0649 | Cell Name | HeLa.P3 |
|---|---|---|---|
| Profile | Serum free cell line of the famous HeLa. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 31 |
| Identity | available | Tissue for Primary Cancer | uterine cervix |
| Case history | cells were established by Katsuta,H. et.at. by serum free medium. | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epitherial-like | Character | This cell line is surpplied from Riken Genebank for the replacement to the JCRB0606:HeLa.P3(S). |
| Classify | variant | Established by | Katsuta,H. |
| Registered by | Takaoka,T. | Regulation for Distribution | free |
| Comment | Year | 1997 | |
| Medium | DM210 medium can be used although the DM160 medium was originally indicated. Be sure this cell line is the serum free cell culture. Frozen medium FM-1 established by Ohno,T. and destributed from Kyokuto Seiyaku Kogyo.Co. used. | Methods for Passages | Harvest cells by rubber policeman. |
| Cell Number on Passage | 1 x 10^4 cells/sq.cm. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | cervix |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | + | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:9892199 | Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping. Macville M,Schröck E,Padilla-Nash H,Keck C,Ghadimi BM,Zimonjic D,Popescu N,Ried T Cancer Res. 1999 Jan 1;59(1):141-50 |
| Pubmed id:2163633 | Cytosol type electrolyte medium suitable for long term growth of human cells with very low membrane potential. Kagawa Y,Takaoka T,Hamamoto T,Ohta T Biochem Biophys Res Commun. 1990 Jun 29;169(3):1217-21 |
| Pubmed id:14404771 | Cultivation of strain HeLa cells (human cervical carcinoma) in the simplified replicate tissue culture. KATSUTA H,TAKAOKA T,KUWABARA H,KUWABARA S Jpn J Exp Med. 1959 Oct;29():493-505 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0649 | Cell Name | HeLa.P3 |
|---|---|---|---|
| LOT No. | 120597 | Lot Specification | distribution |
| Medium | DM160 medium only. No serum added! | Temperature | 37 C |
| Cell Density at Seeding | 1 x 10^4 cells/sq.cm. | Methods for Passages | Cells harvested with scraper (rubber policeman). |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.9x10^6 |
| Viability at cell freezing (%) | 74.0 | Antibiotics Used | free |
| Passage Number | P4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:12,14 D7S820:8,12 D16S539:9,10 VWA:16,18 TH01:7 AM:X TPOX:8,12 CSF1PO:9,10 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Frozen medium FM-1 established by Ohno,T. and destributed from Kyokuto Seiyaku Kogyo.Co. used. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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