JCRB9129 BCE C/D-1b

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9129 Cell Name   BCE C/D-1b
Profile   Bovine corneal endothelial cell line. Other Name   
Animal   bovine Strain   
Genus   Bos Species   taurus
Sex   M Age   adult
Identity    Tissue for Primary Cancer   cornea
Case history   normal Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology   epithelial-like Character   extracellular matrix (ECM) proteins are produced
Classify   normal Established by   Nichokas,A.H. & Bethea,C.L.
Registered by    Regulation for Distribution   none
Comment    Year   1997
Medium   Dulbecco's modified Eagle's medium with 10% calf serum and 5% fetal bovine serum. Methods for Passages   Rinse monolayer with 0.25% trypsin and 0.03% EDTA . Remove the solution and sit at room temperature until the cells detach. 2-3 change per week.
Cell Number on Passage   Split ratio=1/2 - 1/4. Race   
CO2 Conc.   5 % Tissue Sampling   cornea
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2865
Reference
Pubmed id:6500171Effect of extracellular matrix on PC 12 cell shape and dopamine processing.
Bethea CL,Kozak SL
Mol Cell Endocrinol. 1984 Oct;37(3):319-29
Pubmed id:302215Stimulation of corneal endothelial cell proliferations in vitro by fibroblast and epidermal growth factors.
Gospodarowicz D,Mescher AL,Birdwell CR
Exp Eye Res. 1977 Jul;25(1):75-89

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9129 Cell Name   BCE C/D-1b
LOT No.   080297 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum and 5% fetal bovine serum. Temperature   37 C
Cell Density at Seeding   1-2x10^4 cells/sq.cm. Methods for Passages   Cells were harvested by treatment with 0.25% trypsin and 0.02% EDTA for 5 minutes at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   P78 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   under examination
Chromosome Mode    Chromosome Information   under examination
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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