-Detailed Information [JCRB0123]-

JCRB0123 KO52

Cell information

Important Notice(s)

[Note for centrifugation of cell suspension: the size of this cell is very small｜細胞遠心時の注意：細胞のサイズ小]

[Note for centrifugation of cell suspension: the size of this cell is very small.]

The size of this cells is very small, and therefore the cells may not be satisfactorily sedimented by centrifugation.

When you collect the cells by centrifugation, please check that the pellet of cells is firmly formed and that the supernatant is clear.

Depending on the volume of cell suspension and condition, the centrifugation at 1200 - 1500 rpm (about 250 - 400 g) for 5 - 10 min may be required.

[本細胞の遠心時の注意：細胞のサイズが小さいです]

本細胞のサイズはきわめて小さいため、遠心時に十分に沈降しないことがあります。

細胞を遠心により集める際には、ペレットが確実に形成され上清が透明であることを確認してください。

細胞浮遊液の量や条件によって、1200 - 1500 rpm、5 - 10分の遠心が必要とされます。

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Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0123	Cell Name	KO52
Profile	leukemia, acute myeloblastic	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	46
Identity	available	Tissue for Primary Cancer	hemo-lymphocytic
Case history	acute myeloblastic leukemia/M2	Metastasis	No
Tissue Metastasized		Genetics	CD12,CD33,CD2,CD5,CD7,HLA-DR. chr=17p-, MDR1 exp.
Life Span	infinite	Crisis PDL
Morphology	lymphocyte-like	Character	N-ras mutation: G->C in codon 13.
Classify	tumor	Established by	Abo,J. & Inokuchi,K. et.al.
Registered by	Inokuchi,K.	Regulation for Distribution
Comment		Year	1997
Medium	Alpha-MEM with 10% heat inactivated fetal calf serum .	Methods for Passages	Simple dilution (suspension culture).
Cell Number on Passage	2.5 x 10^5 cells/ml	Race
CO2 Conc.	5 %	Tissue Sampling	bone marrow
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1321
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	1330932

Reference
Pubmed id:7693050	p53 and N-ras mutations in two new leukemia cell lines established from a patient with multilineage CD7-positive acute leukemia. Abo J,Inokuchi K,Dan K,Nomura T Blood. 1993 Nov 1;82(9):2829-36

Images

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

11242010
081497
04082016
05272016
07142021
08182025

Cell No.	JCRB0123	Cell Name	KO52
LOT No.	11242010	Lot Specification	distribution
Medium	Alpha-MEM (minimum essential medium alpha) with 10% heat-inactivated fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	approx. 1 x 10^5 cells/ml	Methods for Passages	Simple dilution
Doubling Time	52 - 58 hrs	Cell Number in Vial (cells/1ml)	2.3 x 10^6 cells/ml
Viability at cell freezing (%)	88.5	Antibiotics Used	free
Passage Number	Unkown (9 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - alpha-MEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0123	Cell Name	KO52
LOT No.	081497	Lot Specification	distribution
Medium	Alpha-MEM with 10% heat inactivated fetal calf serum (JRH Bio Sciences 4B2013).	Temperature	37 C
Cell Density at Seeding	2 x 10^5 cells/ml	Methods for Passages	Simple dilution (suspension culture).
Doubling Time	See graph.	Cell Number in Vial (cells/1ml)	3.2x10^6
Viability at cell freezing (%)	92.0	Antibiotics Used	free
Passage Number	P6*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:11 D13S317:8,12 D7S820:9,10 D16S539:9,11 VWA:16 TH01:6,7 AM:XY TPOX:8 CSF1PO:11,12
Adhesion	No	Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0123	Cell Name	KO52
LOT No.	04082016	Lot Specification	distribution
Medium	Alpha-MEM with 10% heat inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	approx. 5 x 10^5 cells/mL	Methods for Passages	Simple dilution (suspension culture). In the first cultivation, do not excessivly dilute. Please seed all cells to one T-25 flask (or one 6 cm dish).
Doubling Time	approx. 61 hrs.	Cell Number in Vial (cells/1ml)	6.9 x 10^6
Viability at cell freezing (%)	85	Antibiotics Used	free
Passage Number	Unknown (14 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:11 D13S317:8,12 D7S820:9,10 D16S539:9,11 VWA:16 TH01:6,7 AM:X,Y TPOX:8 CSF1PO:11,12
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - alpha-MEM	CO2 Conc.	5%
Viability immediately after thawing (%)	85	Additional information	Don't dilute too much on the first culture so the cell death may be easily caused.

Cell No.	JCRB0123	Cell Name	KO52
LOT No.	05272016	Lot Specification	distribution
Medium	Alpha-MEM with 10% heat inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091).	Temperature	37 C
Cell Density at Seeding	approx. 5 x 10^5 cells/mL	Methods for Passages	Simple dilution (suspension culture).
Doubling Time	approx. 32 hrs.	Cell Number in Vial (cells/1ml)	4.2 x 10^6
Viability at cell freezing (%)	81	Antibiotics Used	free
Passage Number	Unknown (13 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene	NT
Medium for Freezing	5% DMSO, 20% HI-FBS - alpha MEM	CO2 Conc.	5%
Viability immediately after thawing (%)	81	Additional information

Cell No.	JCRB0123	Cell Name	KO52
LOT No.	07142021	Lot Specification	distribution
Medium	Alpha-MEM with 10% fetal bovine serum (FBS; Biowest, Cat. # S1820-500)	Temperature	37 C
Cell Density at Seeding	1.4 x 4.7 x 10^5 cells/mL	Methods for Passages	Simple dilution (suspension culture).
Doubling Time	approx. 2 days.	Cell Number in Vial (cells/1ml)	2.8 x 10^6
Viability at cell freezing (%)	92.0	Antibiotics Used	free
Passage Number	Unknown (10 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - alpha-MEM	CO2 Conc.	5%
Viability immediately after thawing (%)	90.8	Additional information

Cell No.	JCRB0123	Cell Name	KO52
LOT No.	08182025	Lot Specification	distribution
Medium	Alpha-MEM with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K).	Temperature	37 C
Cell Density at Seeding	1 - 3 x 10^5 cells/mL	Methods for Passages	Simple dilution (suspension culture), Split 1:2 - 1:3.
Doubling Time	approx. 2 days.	Cell Number in Vial (cells/1ml)	5.3 x 10^6
Viability at cell freezing (%)	95.2	Antibiotics Used	free
Passage Number	Unknown (17 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - alpha MEM	CO2 Conc.	5%
Viability immediately after thawing (%)	92.5	Additional information	The label of this vial: KO52 <---(Name of cell line). JCRB0123 <---(Catalog #). 08182025 <---(Lot #).