JCRB9126 BS-C-1
Cell information
Cell type:general cells (View Pricing Information)
| JCRB No. | JCRB9126 | Cell Name | BS-C-1 |
|---|---|---|---|
| Profile | Kidney cell line of the african green monkey. | Other Name | |
| Animal | monkey, African green | Strain | |
| Genus | Species | ||
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | kidney | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | kidney | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | This cell line is a suitable host for transfection experiments of SV40 vectors. |
| Classify | normal | Established by | Hopps,H.E. et al. |
| Registered by | JCRB | Regulation for Distribution | |
| Comment | Prepared from CCL 26. | Year | 1997 |
| Medium | Eagle's minimal essential medium with non-essential amino acids and 10% calf serum | Methods for Passages | Cells are treated with 0.02% EDTA and 0.25% trypsin. |
| Cell Number on Passage | split ratio=1/2 - 1/4 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | kidney |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:3224379 | A novel 53 kDa actin binding protein from porcine brain--further biochemical and immunological characterization. Maekawa S,Ohta K,Sakai H Cell Struct Funct. 1988 Oct;13(5):373-85 |
| Pubmed id:3465720 | Purification and properties of epithelial growth inhibitor (EGI) from human platelets: its separation from type beta transforming growth factor (TGF-beta). Huang W,Kimura T,Mashima K,Miyazaki K,Masaki H,Yamashita J,Horio T J Biochem. 1986 Sep;100(3):687-96 |
| Pubmed id:6304690 | Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells. Razzaque A,Mizusawa H,Seidman MM Proc Natl Acad Sci U S A. 1983 May;80(10):3010-4 |
| Pubmed id:4203458 | A comparison of three in vivo assays for cell tumorigenicity. Petricciani JC,Wallace RE,McCoy DW Cancer Res. 1974 Jan;34(1):105-8 |
| Pubmed id:4350227 | A temperature-sensitive mutant of simian virus 40 affecting transforming ability. Kimura G,Dulbecco R Virology. 1973 Apr;52(2):529-34 |
| Pubmed id:14215895 | THE SENSITIVITY OF GRIVET MONKEY KIDNEY CELL LINE BS-C-1 FOR PROPAGATION AND ISOLATION OF CERTAIN HUMAN VIRUSES. SCHMIDT NJ,LENNETTE EH,SHON CW,DENNIS J Am J Public Health Nations Health. 1964 Sep;54():1522-30 |
| Pubmed id:14071033 | BIOLOGIC CHARACTERISTICS OF A CONTINUOUS KIDNEY CELL LINE DERIVED FROM THE AFRICAN GREEN MONKEY. HOPPS HE,BERNHEIM BC,NISALAK A,TJIO JH,SMADEL JE J Immunol. 1963 Sep;91():416-24 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB9126 | Cell Name | BS-C-1 |
|---|---|---|---|
| LOT No. | 032797 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 0.1mM non-essential amino acids with 10% fetal calf serum/PVF1 | Temperature | 37 C |
| Cell Density at Seeding | 1.5x10^4 cells/sq.cm. | Methods for Passages | Cells harvested with 0.25% trypsin and 0.02% EDTA for 5 minutes at room temperature. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.8x10^6 |
| Viability at cell freezing (%) | 91.0 | Antibiotics Used | free |
| Passage Number | P49 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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