JCRB9124 M.dunni(Clone III8C)
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9124 | Cell Name | M.dunni(Clone III8C) |
|---|---|---|---|
| Profile | normal, support the growth of all 4 classes of MuLV | Other Name | M.dunni |
| Animal | pygmy mouse | Strain | |
| Genus | Mus | Species | dunnis |
| Sex | F | Age | |
| Identity | Tissue for Primary Cancer | tail | |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | Cloned at P14 using Terasaki plates. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | Supports the growth of all 4 classes of MuLV. |
| Classify | normal | Established by | Lander,M.R.&Chattopadhyay,S.K. |
| Registered by | Lander,M.R.&C.S.K. | Regulation for Distribution | ATCC(memo:cells looks like fibroblastic shape at the begining of the culture.) |
| Comment | Year | 1994 | |
| Medium | McCoy's 5a medium with 10% fetal bovine serum. RPMI1640 or DMEM with 10% fetalbovine serum (DMSO). | Methods for Passages | See reference {4089} for the details of the medium and culture method for the assay of virus infection. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | tail |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:6092693 | A Mus dunni cell line that lacks sequences closely related to endogenous murine leukemia viruses and can be infected by ectropic, amphotropic, xenotropic, and mink cell focus-forming viruses. Lander MR,Chattopadhyay SK J Virol. 1984 Nov;52(2):695-8 |
| Pubmed id:7094696 | Asynchronous replication of constitutive heterochromatin on X chromosomes in female Mus dunni. Possible influence of facultative heterochromatin on the adjacent constitutive heterochromatin. Sen S,Sharma T Chromosoma. 1982;85(1):119-26 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB9124 | Cell Name | M.dunni(Clone III8C) |
|---|---|---|---|
| LOT No. | 110894 | Lot Specification | distribution |
| Medium | McCoy 5A medium with 10% fetal bovine serum (Hyclone 11151031), McCoy5A(ICN Flow, 1xMcCoy's 5A) | Temperature | 37 C |
| Cell Density at Seeding | 1.5-3x10^4 cells/sq.cm. | Methods for Passages | Wash cells with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 5-15min at 37C or Rm.tmp. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.8x10^6 |
| Viability at cell freezing (%) | 90.1 | Antibiotics Used | free |
| Passage Number | P16 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD,LDH,NP |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |