JCRB0746 YKG-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0746 Cell Name   YKG-1
Profile   glioblastoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   53
Identity   available Tissue for Primary Cancer   neural
Case history   a malignant brain tumor. Signal found by CT scan at right temporo-occipital region. glioblastoma. Metastasis   No
Tissue Metastasized    Genetics   GFA and S-100 protein identified. HSR on marker chromosome identified. alpha- & behta-TGFs expressed
Life Span   infinite Crisis PDL   
Morphology   neuron-like Character   human glioma cell line established from a surgical specimen of a patient with glioblastoma
Classify   tumor Established by   Sugano,H.
Registered by   Sugano,H. Regulation for Distribution   Not applied.
Comment    Year   1991
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   4x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1796
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 687592
Reference
Pubmed id:2589023Establishment of a human glioma cell line bearing a homogeneously staining chromosomal region and releasing alpha- and beta-type transforming growth factors.
Kanno H,Kuwabara T,Shinonaga M,Chang CC,Tanaka Y,Sugio Y,Morita H,Yasumitsu H,Umeda M,Nagashima Y
Acta Neuropathol. 1989;79(1):30-6

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0746 Cell Name   YKG-1
LOT No.   11012018 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   5 - 10 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   93.3 Antibiotics Used   free
Passage Number   P49 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   93.3 Additional information   
Cell No.   JCRB0746 Cell Name   YKG-1
LOT No.   07222024 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 25 hrs. Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   96.2 Antibiotics Used   free
Passage Number   P52 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   87.5 Additional information   
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