JCRB9123 B95-8

Cell information

Important Notice(s)

B95-8細胞についての注意

Cell type：virus production cells (View Pricing Information)

JCRB No.   JCRB9123 Cell Name   B95-8
Profile   Monkey(tamarin) lymphocyte cell line producing EBV. Other Name   
Animal   cotten-top tamarin Strain   
Genus   Saguinas Species   oedipus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   leukocytes Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   EBV transformed lymphocyte cell line. Cells are releasing EBV (herpesvirus 4) virus.
Classify   transformed Established by   Caputo,et.al.
Registered by    Regulation for Distribution   The B95-8 cell line must be handled in the BSL-2 labotory since EBV can infect primates.
Comment   Marmoset, used to be described in the animal name section, was a mistake and corrected to the name, cotton-top tamarin with the scientific name. This cell line is used as a source of EBV. Year   1993
Medium   RPMI1640 medium with 10% heat inactivated fetal calf serum. Methods for Passages   Simple dilution.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1953
Reference
Pubmed id:11778691Molecular cytogenetic characterization of the EBV-producing cell line B95-8 (Saguinus oedipus, Platyrrhini) by chromosome sorting and painting.
Müller S,Neusser M,O'Brien PC,Wienberg J
Chromosome Res. 2001;9(8):689-93
Pubmed id:6086497Presence of retrovirus in the B95-8 Epstein-Barr virus-producing cell line from different sources.
Tumilowicz JJ,Gallick GE,East JL,Pathak S,Trentin JJ,Arlinghaus RB
In Vitro. 1984 Jun;20(6):486-92
Pubmed id:4357683Comparison of the yield of infectious virus from clones of human and simian lymphoblastoid lines transformed by Epstein-Barr virus.
Miller G,Lipman M
J Exp Med. 1973 Dec 1;138(6):1398-412
Pubmed id:4346033Release of infectious Epstein-Barr virus by transformed marmoset leukocytes.
Miller G,Lipman M
Proc Natl Acad Sci U S A. 1973 Jan;70(1):190-4
Pubmed id:4333982Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes.
Miller G,Shope T,Lisco H,Stitt D,Lipman M
Proc Natl Acad Sci U S A. 1972 Feb;69(2):383-7

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9123 Cell Name   B95-8
LOT No.   07042014 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO catalog no.11875) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.99x10^5
Viability at cell freezing (%)   98.5 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9123 Cell Name   B95-8
LOT No.   072795 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum (ICN flow 1010183) Temperature   37 C
Cell Density at Seeding   3.9x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.7x10^6
Viability at cell freezing (%)   95.9 Antibiotics Used   free
Passage Number   P3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   under examination
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9123 Cell Name   B95-8
LOT No.   012593 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   2.8x10^5/m Methods for Passages   simple dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   91.1 Antibiotics Used   free
Passage Number   P2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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